Selection of the microorganism and polysaccharide:
Selection of microorganisms used in the study was
based on four criteria: pathogenicity, cell size, type ofpolymer produced and competition with other soil
microorganisms. As manure will be spread on
agricultural land after the storage period, contaminated
manure could spread the disease to healthy animals and
thus, the selected microorganism must be nonpathogenic.
Cells smaller than the average porediameter of the soil (the average pore diameter of
agricultural soils is 7-15 μm) are desirable. Non-soluble
polysaccharide is required in order to form stable
sealing. Arthrobacter and Bacillus species are the most
dominant soil microorganisms[14]. Thus, selected
microorganisms must be able to compete with these as
well as other microorganisms such as yeast and fungi.
Levan was selected as the polysaccharide and the
literature was reviewed for levan producing bacteria
(Table 1). The biological and biochemical
characteristics of Bacillus licheniformis (Table 2)
indicated that the microorganisms would be suitable for
the production of β-D Fructofuranose (levan) from
sucrose.

微生物和多聚糖的选择
微生物和多聚糖的选择有四个标准：致病性、孔眼大小、聚合体畸形伸长的类型和其他泥土微生物的竞争。由于粪便等肥料将会在储存期过后铺在农业地面上，污染的肥料将可能会向动物传播疾病，因此，经过选择的微生物必须是非致病的。土壤中微小的细胞比普通的微孔直径（耕种土壤中普通的微孔直径是7-15 μm）更可取。为了形成稳定的猎捕海豹业，不可容的多聚糖是必须的。节杆菌和杆菌物种是最占支配地位的泥土微生物。因此，经由选择的微生物必须能够和它们竞争当然也包括其他的微生物例如发酵物和真菌类。果聚糖曾经被选作为多聚糖，并且文学作品曾经因为国剧唐曾生产多聚糖而为之写评论。生物学上和生物化学上特有的杆菌显示微生物可能适合从蔗糖里面提炼出β-D呋喃果糖（果聚糖）。