American Journal of Biotechnology and Biochemistry 3 (2): 47-54, 2007
ISSN 1553-3468
© 2007 Science Publications
Corresponding Author: Abdel Ghaly, Department of Process Engineering and Applied science, Dalhousie University,
Halifax, Nova Scotia, Canada B3J2X4, Tel: 902494-6014, Fax: 9024232423
47
Production of Levan by Bacillus licheniformis for Use as a Soil Sealant
in Earthen Manure Storage Structures
Ghaly, A.E., F. Arab, N.S. Mahmoud and J. Higgins
Department of Process Engineering and Applied Sciences
Dalhousie University, Halifax, Nova Scotia, Canada B2J 2X4
Abstract: Manure application is not permitted on frozen land in Canada and therefore, manure
management and storage are the primary issues facing the agri-food industry. Low-cost, effective and
environmentally safe earthen manure storage (EMS) facilities will lower costs and help make the
livestock industry more competitive and efficient. The goal of this study was to develop a biological
sealing technology for earthen manure storages. The results showed that it is feasible to use a growing
culture of Bacillus licheniformis to produce a non viscous water insoluble levan. Levan can only be
produced by Bacillus licheniformis during the growth mode. No levan was produced during the death
phase. About 0. 36 g of levan was produced per gram of sucrose which is 91. 1% of theoretical yield.
The polymer can be used as a plugging agent to plug the pores of high permeability soils. From the
biological and biochemical characteristics of the Bacillus licheniformis, it appears that the organism is
capable of producing levan from sucrose under most field and soil conditions. As a soil organism,
Bacillus licheniformis should be able to compete with most common soil species such as Arthrobacter
and Bacillus. The bacteria could be grown either in the non-polysaccharide producing mode or in the
polysaccharide producing mode. The first would permit distribution of the bacteria to the lower soil
layers but would delay the production of the polysaccharide due to the lag period required to produce
the enzyme (levansucrase). Upon production of levan, pore spaces would close and hence, the
hydraulic conductivity would be substantially reduced.
Keywords: Mnure, soil, biological sealing, bacteria, polysaccharide, plugging agent, levan

美国生物工艺学与生物化学杂志 3（2）：47-54，2007
刊号 1553-3468
科技出版社 2007
原著：阿卜杜勒 加利，达尔豪西大学工艺技术与应用科学系
加拿大新斯科舍省哈立法斯 邮编B3J2X4，电话902494-6014 传真902423242347

地衣芽孢杆菌的产物左聚糖在地球肥料存储结构中被用做土样密封剂
加利，A.E.,F.啊拉伯,N.S.穆罕默德，J.希金斯
达尔豪西大学工艺技术与应用科学系，加拿大新斯科舍省哈立法斯 邮编B3J2X4

摘要：在加拿大冰天雪地的环境中施用肥料是被禁止的，所以，肥料的管理和储存是食品工业面对的首要
问题。低成本的，有效的，对环境安全的世界肥料储存设备将会降低成本并且可以帮助畜牧业变的更有竞
争力和效率。本研究的目的在于发展世界肥料储存生物密封技术。结果显示通过培养生长地衣芽孢杆菌来
制作出一种可溶于稠化水的左聚糖是可行的。左聚糖只能通过地衣芽孢杆菌在生长状态期间才能生产出来
。没有一个左聚糖是在死亡阶段生产出来的。每克蔗糖可制造出0.36克左聚糖，是理论产率的91.1%。
该聚合物可以做为封堵剂来封堵高渗透性土壤的每一处缝隙。从地衣芽孢杆菌的生物特性和生化特性可以
得知，生物体可以在大多数土地和土壤环境下从蔗糖中制造出左聚糖。以土壤生物为例，地衣芽孢杆菌与
最普通土壤中的微生物竞争，特别是节杆菌属和芽胞杆菌属。细菌即可以在单糖的培养环境下生长又可以
在多糖的环境下生长。首先细菌可以在低层土壤中分布，但是由于迟滞期肯定会产生酶（果聚糖生成酶）
这将会多糖的产物延期。使用左聚糖产物，缝隙空间将被封住，因此，渗透系数将会相当大地减少。

关键词：肥料，土壤，生物密封，细菌，多糖，封堵剂，左（果）聚糖


完全手工翻译,我不是学这个门类的,有些关键词可能翻译的不够准确,请检查后再替换.
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